Figure 4.

MSCA1 (mesenchymal stem cell antigen 1) expression in native SVF cells is associated with cellular respiration in vitro. (A) The OCR of the investigated specific parameters of the mitochondrial respiratory chain did not differ between pre-passaged SVF cells of lean children and children with overweight/obesity (owt/ob). Bar plots show quantitative data of n = 23 children (mean ± SEM). (B) High MSCA1 expression in native SVF cells is associated with a higher OCR in pre-passaged cells. Graph depicts the averaged seahorse measurement (mean ± SEM) of n = 9 SVF cell samples with high compared to n = 14 samples with low MSCA1 expression. (C) MSCA1 expression in native SVF cells correlates with specific parameters of the mitochondrial respiratory chain, ie, basal respiration, proton leak, maximal respiration and spare capacity. (D) The gene expression of marker genes for beige/brown adipogenesis (PRDM16, PGC1A, UCP1) and the regulator of the futile creatine cycle CKB showed no significant correlation with MSCA1 expression in native SVF cells. Pearson correlation coefficient R and p value are given in each scatter plot. Significant p values (P < 0.05) are indicated in bold. SVF samples from lean children are represented as open circles and children with overweight/obesity as closed circles. Abbreviations: ATP, adenosine triphosphate; CKB, creatine kinase B; FCCP, carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone; OCR, oxygen consumption rate; PGC1A, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PRDM16, PR domain containing 16; UCP1, uncoupling protein 1.