Table 4.
MSCA1 expression | ||||||
---|---|---|---|---|---|---|
Native SVF cells | Prepassaged SVF cells | |||||
Functional parameters of mitochondrial respiration chain | Low (≤0.228) n = 14 |
High (>0.228) n = 9 |
P | Low (≤0.180) n = 15 |
High (>0.180) n = 8 |
P |
Basal respiration, pmol/min/cell count | 39.3 ± 2.1 | 48.6 ± 3.6 | 0.060 | 42.0 ± 2.3 | 41.9 ± 2.9 | 0.978 |
ATP production, pmol/min/cell count | 34.7 ± 1.8 | 40.3 ± 2.1 | 0.057 | 37.1 ± 1.9 | 36.5 ± 2.4 | 0.835 |
Proton leak, pmol/min/cell count | 4.7 ± 0.4 | 5.8 ± 0.8 | 0.187 | 4.9 ± 0.5 | 5.5 ± 0.7 | 0.530 |
Maximal respiration, pmol/min/cell count | 120.5 ± 7.9 | 163.4 ± 12.8 | 0.006 | 140.0 ± 12.0 | 132.2 ± 7.0 | 0.657 |
Spare capacity, pmol/min/cell count | 65.9 ± 5.5 | 96.9 ± 10.5 | 0.009 | 80.6 ± 8.8 | 73.4 ± 6.5 | 0.585 |
Non-mitochondrial OCR, pmol/min/cell count | 15.3 ± 1.5 | 20.4 ± 1.9 | 0.044 | 17.4 ± 1.7 | 16.9 ± 1.8 | 0.856 |
CD36 expression | ||||||
Native SVF cells | Prepassaged SVF cells | |||||
Functional parameters of mitochondrial respiration chain | Low(≤0.120) n = 14 |
High (>0.120) n = 9 |
P | Low (≤0.018) n = 13 |
High (>0.018) n = 10 |
P |
Basal respiration, pmol/min/cell count | 43.8 ± 2.7 | 39.2 ± 1.2 | 0.219 | 42.2 ± 2.6 | 41.7 ± 2.4 | 0.891 |
ATP production, pmol/min/cell count | 38.3 ± 2.3 | 34.7 ± 1.1 | 0.230 | 37.2 ± 2.2 | 36.6 ± 1.9 | 0.844 |
Proton leak, pmol/min/cell count | 5.4 ± 0.6 | 4.6 ± 0.5 | 0.301 | 5.1 ± 0.5 | 5.1 ± 0.7 | 0.915 |
Maximal respiration, pmol/min/cell count | 146.1 ± 12.2 | 123.7 ± 6.8 | 0.183 | 143.9 ± 11.8 | 128.7 ± 10.5 | 0.366 |
Spare capacity, pmol/min/cell count | 84.6 ± 9.1 | 67.9 ± 5.6 | 0.189 | 84.3 ± 8.9 | 69.9 ± 7.6 | 0.251 |
Nonmitochondrial OCR, pmol/min/cell count | 17.7 ± 1.5 | 16.5 ± 2.3 | 0.645 | 17.4 ± 1.6 | 17.1 ± 2.2 | 0.927 |
Significant P-values (P < 0.05) are indicated in bold. Gene expression of n = 23 samples is grouped into high (>mean expression) and low (≤mean expression).
Abbreviations: ATP, adenosine triphosphate; CD36, cluster of differentiation 36; MSCA1, mesenchymal stem cell antigen 1; OCR, oxygen consumption rate; SVF, stroma vascular fraction.