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. 2022 Jan 4;9:803052. doi: 10.3389/fbioe.2021.803052

FIGURE 3.

FIGURE 3

GDF11 promotes axonal growth of DRG cells in vitro. (A and B). After DRG cells were cultured with recombinant GDF11 for 7 days, axon length was measured by NF200 immunofluorescence. (C). Western analysis showed increased phosphorylation of Smad1/5/8 when DRG cells were treated with GDF11. (D). Western analysis showed increased phosphorylation of Smad2/3 when DRG cells were treated with GDF11. Statistical analysis was based on at least three different biological samples and three technical replicates, p < 0.05 was considered to be statistically significant. Representative images are shown.