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. 2022 Jan 4;12:798938. doi: 10.3389/fphar.2021.798938

FIGURE 2.

FIGURE 2

Viability of RPE cells exposed to tocopherols and αTr for either four or 24 h followed by exposure to tBHP (500 μM) for 24 h measured using the calcein-acetomethoxy ester (AM) assay. (A) Calcein-AM assay results of hTERT-REP cells exposed to 4-h of vitamin E components or vehicle control (VC) followed by 24-h tBHP exposure. Light gray bars are conditions with no tBHP while dark gray bars are conditions with 500 μM tBHP. The 4-h αT exposure offered no protection of hTERT-RPE cells against tBHP. (B) Calcein-AM assay results of hTERT-REP cells exposed to vitamin E components (or VC) for 24-h followed by 24-h tBHP exposure. Light gray bars are conditions with no tBHP while dark gray bars are conditions with 500 μM tBHP. At 24 h, all vitamin E components protected (≥85% protection) hTERT-RPE cells against tBHP. One-way ANOVA analysis with post hoc Bonferroni correction was performed to determine differences between groups. **p = ≤ 0.01 versus vehicle control (VC)-tBHP. Calcein assay data from each data set are from an average of four samples. Data for 4-h tocopherol and 24-h tocopherol groups were compared using a one-way ANOVA with Tukey’s post hoc test to determine whether tocopherol protection was time-dependent.