FIGURE 5.

Effect of αT, γT, Oxidative Stress and Protein Synthesis on ZO-1 Expression. (A) The image panel shows ZO-1 IR (green) and the nucleus (blue) in the presence or absence of αT or γT, with or without tBHP and with or without CHX. Images are 63x magnification and the scale bar is 50 μm. (B) Optically magnified images demonstrating condensed ZO-1 IR (white arrows) and nuclear shrinkage (white arrows) observed under conditions of oxidative stress. (C) Quantitative graphical data calculated from images revealed that tBHP alone, but neither αT nor γT alone, induced ZO-1 expression (ZO-1 IR, green) by 2.6-fold. The presence of αT reduced the effect of tBHP on ZO-1 induction, but it failed to reach statistical significance. The presence of γT, however, completely prevented tBHP’s effect on ZO-1 induction. Furthermore, inhibition of protein synthesis with CHX did not significantly affect tBHP-mediated ZO-1 induction nor αT-tBHP’s effect on ZO-1 IR. CHX, however, completely prevented γT’s reversal of tBHP-mediated ZO-1 induction. Microfluorimetric analyses are from an average of three images. *p = ≤0.05, **p = ≤ 0.01 with tBHP versus the same condition without tBHP as determined by one-way ANOVA analysis with Bonferroni post hoc test.