TABLE 2.
Virulence and antimicrobial resistance genes other than bla identified in 30 ESBL-producing Escherichia coli isolates obtained from Finnish patients collected in the Eastern Finland healthcare district during 2018–2020.
| Virulence factors |
||||||||
| Sample | Acquired resistance genes other than bla | Fosfomycin resistance mutations | Quinolone resistance mutations | Adherence | Invasion | Iron uptake | Toxins | Effector delivery system |
| D1 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A), dfrA17 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fdeC, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D2 | aph(3″)-Ib, aph(6)-Id, mph(A), sul2, dfrA14 | Not found | Not found | fimA, fimB, fimC, fimD, fimE, fimF, fimG, fimH, fimI, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, ibeA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entD, entE, entS, fdeC, fepA, fepB, fepC, fepD, fepG, fes | pic, set1A, set1B, vat | |
| D3 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A), dfrA17 | Not found | gyrA (D87N), gyrA (S83L), parC (S80I) | fdeC, fimA, fimB, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entD, entE, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | espL1, espL4, espR1, espX1, espX4, espX5, espY1, espY2, espY3, espY4 |
| D4 | tet(M) | Not found | marR (S3N) | fdeC, fimA, fimB, fimC, fimD, fimE, fimF, fimG, fimH, fimI, focC, focD, focF, focI, papB, papC, papD, papF, papI, papJ, papK, sfaA, sfaB, sfaC, sfaD, sfaE, sfaF, sfaG, sfaH, sfaS, sfaY, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, ibeA, kpsD, kpsM, kpsT, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entD, entE, entS, fepA, fepB, fepC, fepD, fepG, fes, iroB, iroC, iroD, iroE, iroN | cnf1, hlyA, hlyB, hlyC, hlyD, vat | |
| D5 | Not found | uhpT (E350Q) | gyrA (D87N), gyrA (S83L), marR (S3N), parC (S80I), parE (L416F) | fdeC, fimA, fimB, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/ec, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, kpsT, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat, vat | |
| D6 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A), dfrA17 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimB, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D7 | aac(6′)-Ib-cr | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papC, papD, papF, papG, papJ, papK, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | cnf1, hlyA, hlyB, hlyC, hlyD, sat | |
| D8 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A), dfrA17 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdec, fimA, fimB, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat, vat | |
| D9 | Not found | ptsI (V25I), uhpT (E350Q) | gyrA (S83L), parE (I529L) | fdeC, fimA, fimB, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D10 | aadA2, mph(A), sul1, tet(A), dfrA12 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papC, papD, papF, papG, papJ, papK, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | hlyA, hlyB, hlyC, hlyD, sat | |
| D11 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A), dfrA17 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/eC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D12 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A) | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, yagV/ecpE, yagW/ecpD, yagX/eC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D13 | Not found | Not found | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/eC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D14 | aadA2, aph(3″)-Ib, aph(6)-Id, mph(B), cmlA1, sul1, sul2, tet(A), dfrA1 | Not found | gyrA (D87N), gyrA (S83L), parC (S80I) | fdeC, fimA, fimB, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papC, yagV/ecpE, yagW/ecpD, yagX/eC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | ompA | entB, entC, entD, entE, entS, fepA, febB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | astA, east1 | espX1, espX4, espX5 |
| D15 | Not found | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/eC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D16 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A), dfrA17 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/eC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D17 | Not found | Not found | marR (S3N) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, yagV/ecpE, yagW/ecpD, yagX/eC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, ibeA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entS, fepA, fepB, fepC, fepD, fepG, fes | pic, set1A, set1B, vat | |
| D18 | Not found | uhpT (E350Q) | Not found | fimA, fimB, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papX, sfax | aslA, kpsD, kpsM, kpsT, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iucD, iutA | sat | espL1, espR1, espX1, espX4, espY2, espY4 |
| D19 | Not found | Not found | Not found | fdeC, fimA, fimB, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papC, papD, papG, papI, papJ, papK, yagV/ecpE, yagW/ecpD, yagX/eC, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entD, entE, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | hlyA, hlyB, hlyC, hlyD, sat | espL1, espL4, espX1, espX4, espX5, espY2, espY3, espY4 |
| D20 | aph(3″)-Ib, aph(6)-Id, sul2, tet(A) | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfax, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D21 | Not found | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfax, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D22 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A), dfrA17 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D23 | aadA2, mph(A), sul1, tet(A), dfrA12 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papC, papD, papF, papG, papJ, papK, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | hlyD, sat | |
| D24 | aac(6′)-Ib-cr | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D25 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A), dfrA17 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfax, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D26 | Not found | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D27 | aadA2, mph(A), sul1, tet(A), dfrA12 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papC, papD, papF, papG, papJ, papK, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | cnf1, hlyA, hlyB, hlyC, hlyD, sat | |
| D28 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A), dfrA17 | ptsI (V25I), uhpT (E350Q) | gyrA (S83L), parE (I529L) | afaA, afaB-I, afaC-I, afaC-III, afaD, daaA, daaC, daaD, daaF, draA, draB, draC, draD, draP, fdeC, fimA, fimB, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entS, fepA, fepB, fepC, fepD, fepG, fes | hlyA, hlyB, hlyC, hlyD | |
| D29 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A), dfrA17 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |
| D30 | aadA5, aph(3″)-Ib, aph(6)-Id, mph(A), sul1, sul2, tet(A), dfrA17 | ptsI (V25I), uhpT (E350Q) | gyrA (D87N), gyrA (S83L), parC (E84V), parC (S80I), parE (I529L) | fdeC, fimA, fimC, fimD, fimE, fimF, fimG, fimH, fimI, papB, papI, papX, sfaX, yagV/ecpE, yagW/ecpD, yagX/ecpC, yagY/ecpB, yagZ/ecpA, ykgK/ecpR | aslA, kpsD, kpsM, ompA | chuA, chuS, chuT, chuU, chuV, chuW, chuX, chuY, entB, entC, entE, entF, entS, fepA, fepB, fepC, fepD, fepG, fes, iucA, iucB, iucC, iutA | sat | |