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. 2022 Jan 4;12:792361. doi: 10.3389/fmicb.2021.792361

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Copyright © 2022 Yang, Wang, Cheng, Yang, Wu, Huang, Tian, Jia, Liu, Zhu, Chen, Zhao, Zhang, Ou, Mao, Gao, Sun, Yu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect on the other viral genes when downregulated the UL11. (A) mRNA level of UL11, UL16, UL21, gE, ICP22, ICP27, ICP4, US3, and gB. Transfected the shRNA126 into the DEF cells and then infected the cells with 1MOI DPV. After 24 h, the DEF cells were harvested and did the qRT-PCR. ^nsP > 0.05; ***P < 0.001; ****P < 0.0001. (B) Protein expression levels of UL11, UL16, UL21, gE, ICP22, ICP27, US3, and gB when interfering with the UL11 gene. Transfected the shRNA126 into the DEF cells and then infected the cells with 1MOI DPV. After 24 h, the DEF cells were harvested and did the Western blot to undergo grayscale value. ^nsP > 0.05; *P < 0.05.