FIGURE 5.

UL11 can interact with the lipid raft marker, flotillin-1, and prohibition. (A) DEF cells were transfected with EGFP-N1-UL11 for 24 h, then cells were fixed and did the Immunofluorescence analysis. The primary antibody is an anti-FLOT-1 monoclonal antibody, and the secondary antibody is Alexa Fluor 594 Goat anti-Rabbit IgG. The nuclei were stained with DAPI. (B) The co-immunoprecipitation between the UL11 and PHB. HEK 293T cells were transfected with UL11 and pCAGGS for 24 h. Then the samples were collected to do the co-immunoprecipitation. (C) As described in materials and methods, HEK 293T cells were transfected with UL11 and collected 48 h post-transfection. After removed of cell nuclei, cell lysates were subjected to sucrose-gradient centrifugation. Fractions were collected from the top of the tube. Proteins in each fraction were separated by SDS-PAGE and analyzed by Western blotting. (D) As described in Materials and methods, HEK 293T cells were transfected with UL11G2A and fractionated at 48 h post-transfection. After removed of cell nuclei, cell lysates were subjected to sucrose-gradient centrifugation. Fractions were collected from the top of the tube. Proteins in each fraction were separated by SDS-PAGE and analyzed by Western blotting.