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. 2022 Jan 4;12:792162. doi: 10.3389/fmicb.2021.792162

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Copyright © 2022 Muchaamba, Eshwar, Stevens, Stephan and Tasara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Listeria monocytogenes strains display lineage-based differences in zebrafish embryo virulence. Presented are (A) survival curves of zebrafish embryo (n = 10 per strain) infected (500 CFU) with different L. monocytogenes strains representing LI, LII, and LIII. Embryos were monitored for 3 days post-infection. (B–D) Hemolysis, PI-PLC activity, and lysozyme stress tolerance profiles of injected strains are also shown. (D) Dotted line denotes lysozyme resistant strain classification cutoff value. *Significant difference between (B) LI and LIII, (D) LII and the other lineages (P < 0.05). Results are based on three independent biological repeats.