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. 2000 Dec;38(12):4604–4613. doi: 10.1128/jcm.38.12.4604-4613.2000

FIG. 2.

FIG. 2

Schematic representation of the SNAP procedure. The primers used for the PCR step are indicated (●, DIG or FITC label), as is the SNAP oligonucleotide (■, biotin labeled) used for capture of the specific product onto the streptavidin (S)-coated ELISA plate. Final detection is in a standard ELISA format using peroxidase-labeled (POD) antibody.