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. 2022 Jan 4;11:775663. doi: 10.3389/fcimb.2021.775663

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Copyright © 2022 Zhao, Li, Zhou, Pan, Zhao, Yang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NK cells from C.muridarum-infected mice polarized macrophages toward an M1 phenotype in vitro. NK cells from lung of C.muridarum-infected (iNK) or uninfected mice (uNK) were co-cultured with RAW264.7 cells at a 1 to 2 ratio for 24h. After removing NK cells from the culture, iNOS (A), TNF-α (B), ARG-1 (C) and IL-10 (D) gene expression were analyzed in macrophages by qPCR. Data are shown as mean ± S.D and are representative of three independent experiments with similar results. One-way ANOVA was used to determine significant differences between different groups. (n=5) NS, no significant difference; **p < 0.01; ***p < 0.001.