FIGURE 4.

Effect of 6-days cerebral treatment with GW4064 on FXR target gene expression in PVH and energy homeostasis. (A,B) Shp and Bsep mRNA expression in PVH by q-PCR. The values are normalized to cyclophilin. (C) 24 h-oxygen consumption (VO₂). (D) 24 h-carbon dioxide consumption. (E) 24 h-spontaneous locomotor activity (total beam breaks/hour). (F) 24 h-respiratory exchange ratio (RER). (G) 24 h-spontaneous Z-rearing (total beam breaks/hour). Data are mean ± SEM. Unpaired Student’s t test or Two-Way ANOVA followed by Tukey post hoc. Vehicle group is indicated as open circles/bars, GW4064 group as black circles/bars.