FIGURE 6.

Intracerebral treatment with GW4064 functionally impacts the BAT and rectal temperature. (A) Ucp1 mRNA expression in BAT of mice placed at room temperature (23°C) or 4°C. (B) Th mRNA expression in BAT of mice placed at room temperature (23°C) or 4°C. The values are normalized to cyclophilin or 18S. (C) UCP1 and TH protein expression in BAT of mice placed at room temperature (23°C) or 4°C. Results are represented in the form of boxes for illustration purposes. For an experiment, all samples are processed in the same western blot. If different gels were used if the number of wells was insufficient, we took the precaution of introducing one or more common samples within each gel to standardize the results. (D) The bar graphs are the quantification of UCP1 western blots in panel (C). (E) The bar graph is the quantification of TH western blots in panel (C). (F) Rectal temperature was measured at the end of 23°C- or 4 h-cold exposure. Data are mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001, Two-Way ANOVA followed by Tukey post hoc. 23°C group is indicated as open bars, 4°C group as black bars.