TABLE 1.
Bacterial strains, phage, and plasmids used in this study
| Strain | Description | Source |
|---|---|---|
| Escherichia coli | ||
| DH5α | New England Biolabs | (15) |
| S17 | λpir-positive strain | (15) |
| BTH101 | BACTH reporter strain | (25) |
| P. aeruginosa | ||
| PAO1 | Wild type | (56) |
| PAO1 ΔpilA | Clean deletion of pilA from PAO1 | (57) |
| PAO1 ΔintF4 | Clean deletion of intF4 from PAO1 | (8) |
| PAO1 ΔintF4/pfsE | Clean deletion of pfsE from PAO1 ΔintF4 | This study |
| PAO1 ΔPf4 | Clean deletion of the Pf4 prophage from PAO1 | This study |
| PA14 | Wild type | (58) |
| PAK | Wild type | ATCC 25102 |
| E90 | Clinical CF P. aeruginosa isolate | (59) |
| Bacteriophage strains | ||
| Pf4 | Inoviridae | (8) |
| JBD26 | Siphoviridae | (20) |
| CMS1 | Podoviridae | This study |
| DMS3 | Siphoviridae | (60) |
| OMK01 | Myoviridae | (61) |
| LPS-5 | Podoviridae | Felix Biotech |
| Plasmids | ||
| pHERD20T | AmpR, expression vector with araC-PBAD promoter | (49) |
| pHERD30T | GmR, expression vector with araC-PBAD promoter | (49) |
| pHERD30T-PA0717 | pBAD::PA0717 | (19) |
| pHERD30T-PA0718 | pBAD::PA0718 | (19) |
| pHERD30T-PA0719 | pBAD::PA0719 | This study |
| pHERD30T-PA0720 | pBAD::PA0720 | (19) |
| pHERD30T-pfsE | pBAD::pfsE | (19) |
| pHERD30T-PA0722 | pBAD::PA0722 | This study |
| pHERD30T-PA0723 | pBAD::PA0723 | This study |
| pHERD30T-PA0724 | pBAD::PA0724 | This study |
| pHERD30T-PA0725 | pBAD::PA0725 | (19) |
| pHERD30T-PA0726 | pBAD::PA0726 | This study |
| pHERD30T-PA0727 | pBAD::PA0727 | This study |
| pHERD30T-intF4 | pBAD::intF4 | This study |
| pHERD20T-xisF4 | pBAD::xisF4 | (9) |
| pKT25 | BACTH construct | (25) |
| pUT18C | BACTH construct | (25) |
| pHERD20T-pfsE | pBAD::pfsE | This study |
| pHERD20T-pfsE Y16V | pBAD:: pfsE Y16V | This study |
| pHERD20T-pfsE W18A | pBAD:: pfsE W18A | This study |
| pHERD20T-pfsE W20A | pBAD:: pfsE W20A | This study |
| pHERD20T-pfsEY16A/W18A/W20A | pBAD:: pfsE Y16A/W18A/W20A | This study |