TABLE 2.

Primers used in this study

Purpose/name	Sequence (5′–3′)
Cloning
PfsE_p18CFwd	TACGTCTAGAGCTCCGCTATCTCTCGCTGTTCGCGGTAGG
PfsE_p18CRev	TACGGGTACCTCAAACAGTCAGGGAGGCCGCTAGG
PfsE_Y16VFwd	CTGGCCACCGGCGTGGCCTGG
PfsE_Y16VRev	CCAGCCCCAGGCCACGCCGGT
PfsE_W18AFwd	ACCGGCTACGCCGCCGGCTGG
PfsE_W18ARev	TCGATCCAGCCGGCGGCGTAGC
PfsE_W20AFwd	TACGCCTGGGGCGCCATCGACG
PfsE_W20ARev	CTAGGCCGTCGATGGCGCCCCAGG
ΔpfiT primers
attB1-pfiT-UpF	ggggataagtttgtacaaaaaagcaggcttcTTCAACCCGCTCATAGGTT
pfiT-UpR	TCAGGAGTAGAAAGCCATCACATTAAACCTCCTTATTCTGG
PfiT-DownF	TGATGGCTTTCTACTCCTGA
attB2-PfiT-DownR	ggggaccactttgtacaagaaagctgggtaAGCCGCTCAACCCGATCTA
PfiT seq F	CCACACGTTCGCCAGTCACTT
PfiT seq R	AATGCCGGCCACTTCATCGAC
ΔPf4 primers
Pf4-UpF-GWL	tacaaaaaagcaggctTCTGGGAATACGACGGGGGC
Pf4-UpR-GM	tcagagcgcttttgaagctaattcgGATCCCAATGCAAAAGCCCC
Pf4-DnF-GM	aggaacttcaagatccccaattcgCGTCATGAGCTTGGGAAGCT
Pf4-DnR-GWR	tacaagaaagctgggtTGGCAGCAGACCCAGGACGC
pf4-out F	AGTGGCGGTTATCGGATGAC
pf4-out R	TCATTGGGAGGCGCTTTCAT