FIG 2.

PfPX1 and the putative PI3P-binding PX domain are conserved in Plasmodium species. (A) Schematic of PfPX1 and putative domains. TM, transmembrane domain. The two lighter TMs have lower confidence scores than the other four. (B) PfPX1 proteins are conserved across all species of Plasmodium. The phylogenetic distribution demonstrates conservation of PfPX1 based on the E values for the candidate orthologue into the P. falciparum proteome (column reverse BLAST E values), regardless of infectious host (red, primates; blue, rodents; green, birds/reptiles). Relationships of taxa are based on (139). Purple circle represents the phylogenetic placement where the PfPX1 equivalent was acquired during evolution. (C) Schematic of PfPX1 displaying the position of the conserved PX domain and of putative transmembrane domains. Conserved domain structure is shown based on Phyre v2.0. aa, amino acids. Orange box: conserved PX domain. Brown boxes: putative transmembrane domains. (D) Putative PI3P binding motif in the PX domain of PfPX1. The four core binding residues are in red. The residues are numbered according to the full-length PfPX1 protein.