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. 2022 Jan 18;13(1):e03239-21. doi: 10.1128/mbio.03239-21

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Copyright © 2022 Mukherjee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Subcellular localization of PfPX1-GFP. (A) Anti-GFP western blot of mixed stage PfPX1-GFP parasites showing the expression of the fusion protein. (B) Live cell imaging of the PfPX1-GFP parasite line at 3 different stages. Troph: trophozoite. (C) Colocalization of PfPX1-GFP with the endolysosomal marker PfVPS45-mScarlet. White arrow shows the overlapping foci. (D) Colocalization of PfPX1-GFP with PfAP2μ-mScarlet. White arrow shows the overlapping foci. (E) Colocalization of PfPX1-GFP with PfKelch13 (K13) using an anti-PfK13 antibody. White arrow shows the overlapping foci. (F) PfPX1-GFP in red blood cells preloaded with dextran Alexa-594. (F, iv) Colocalization of PfPX1-GFP with LysoTracker. White arrow in iii and iv show the DV extension. Single optical slices shown. (G) Colocalization of PfPX1-GFP with hemoglobin-filled structures. White arrow shows the overlapping foci. (H) Colocalization of PfPX1-GFP with mScarlet-PfRab5a. White arrow shows the juxtaposed foci. (I) Colocalization of PfPX1-GFP with mScarlet-PfRab7. White arrow shows the juxtaposed foci. Dapi: parasite nuclei.