TABLE 1.
Physiological data after 24 h of growth under PHA accumulation conditionsa
| Strain | Total biomass (g/L) | PHA |
Residual biomass (g/L) | (R)-HA concn (g/L) | No. of viable cells (108/ml) | Growth rate (h−1) | |
|---|---|---|---|---|---|---|---|
| % CDW | Concn (g/L) | ||||||
| KT2440 | 1.3 ± 0.1 | 71.7 ± 3.1 | 1.0 ± 0.1 | 0.4 ± 0.0 | 0.2 ± 0.0 | 1.8 ± 0.6 | 0.31 ± 0.02 |
| KT40Z | 1.4 ± 0.0 | 72.3 ± 3.3 | 1.0 ± 0.1 | 0.4 ± 0.0 | 0.0 ± 0.0 | 1.2 ± 0.5 | 0.31 ± 0.01 |
| KT2440 Δpha | 0.6 ± 0.1 | 0.0 ± 0.0 | <0.01 | 0.6 ± 0.1 | 0.0 ± 0.0 | 22.3 ± 4.8 | 0.34 ± 0.00 |
| M1 | 1.3 ± 0.1 | 70.2 ± 3.7 | 0.9 ± 0.1 | 0.4 ± 0.0 | 0.3 ± 0.0 | 4.1 ± 0.3 | 0.30 ± 0.01 |
| M2 | 0.6 ± 0.0 | 0.3 ± 0.4 | <0.01 | 0.6 ± 0.0 | 0.5 ± 0.1 | 16.5 ± 3.1 | 0.29 ± 0.00 |
| M3 | 0.7 ± 0.1 | 0.4 ± 0.4 | <0.01 | 0.7 ± 0.1 | 0.6 ± 0.0 | 16.8 ± 3.1 | 0.31 ± 0.00 |
| M4 | 0.5 ± 0.0 | 0.7 ± 1.0 | <0.01 | 0.5 ± 0.0 | 0.5 ± 0.0 | 15.4 ± 3.6 | 0.35 ± 0.02 |
a
Sodium octanoate (mM) was not detected in the culture supernatant of any of the strains tested.