TABLE 2.
CfiA carbapenemase and associated insertion sequence detection in B. fragilis sensu stricto isolates with phenotypic or genotypic carbapenem resistance determinants
| Strain | 72 h MIC (μg/mL) |
cfiA detection |
IS detection |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Ertapenem | Meropenem | MALDI | PCR | AMRFinder | PCR | ISfinder | Family | Identities | Origin | |
| BJH_13 | >32 | >32 | + | + | cfiA2 | + | IS612B | IS1380 | 1595/1596 | Bacteroides fragilis |
| BJH_14 | >32 | >32 | + | + | cfiA2 | + | IS612B | IS1380 | 1595/1596 | Bacteroides fragilis |
| BJH_140 | 0.5 | 1 | + | + | cfiA | − | Partial ISBf9 | − | − | − |
| BJH_141 | 0.5 | 1 | + | + | cfiA | − | Partial ISBf9 | − | − | − |
| BJH_220 | 0.5 | 1 | + | + | cfiA | − | − | − | − | − |
| IHMA_1 | >32 | >32 | + | + | cfiA4 | + | IS613 | IS1380 | 1595/1595 | Bacteroides fragilis |
| IHMA_2 | 4 | >32 | + | + | cfiA2 | − | None detected | |||
| IHMA_3 | >32 | >32 | + | + | cfiA13 | + | IS942 | IS1380 | 1592/1598 | Bacteroides fragilis |
| IHMA_4 | 32 | >32 | + | + | cfiA2 | + | IS1169 | IS5 | 1301/1317 | Bacteroides fragilis |
| IHMA_5 | 8 | 32 | + | + | cfiA2 | − | − | − | − | − |
| IHMA_6 | >32 | >32 | + | + | cfiA14 | + | IS616 | IS1380 | 1691/1691 | Bacteroides fragilis |
| IHMA_7 | 8 | >32 | + | + | cfiA14 | − | − | − | − | − |
| IHMA_8 | 16 | >32 | − | − | − | − | − | − | − | − |
| IHMA_9 | 1 | 4 | + | + | cfiA14 | − | Partial ISBf9 | − | − | − |
| IHMA_10 | >32 | >32 | + | + | cfiA | + | IS982# | IS982 | − | Barnesiella viscericola |
| IHMA_11 | 8 | >32 | + | + | cfiA2 | − | − | − | − | − |
| IHMA_12 | 1 | 8 | + | + | cfiA | − | − | − | − | − |
| IHMA_13 | 2 | 32 | + | + | cfiA | − | − | − | − | − |
| IHMA_14 | 1 | 8 | + | + | cfiA13 | − | − | − | − | − |
| IHMA_15 | 2 | 16 | + | + | cfiA2 | − | − | − | − | − |
| IHMA_16 | >32 | >32 | + | + | cfiA13 | − | IS614B | IS1380 | 1532/1597 | Bacteroides fragilis |
| IHMA_17 | 16 | >32 | + | + | cfiA4 | + | IS4351 | IS30 | 1155/1155 | Bacteroides fragilis |
| IHMA_18 | >32 | >32 | + | + | cfiA13 | + | IS614 | IS1380 | 1585/1596 | Bacteroides fragilis |
| IHMA_19 | >32 | >32 | + | + | cfiA17 | + | ISBf11 | IS1380 | 1457/1594 | Bacteroides fragilis |
| IHMA_20 | >32 | >32 | + | + | cfiA17 | + | ISBf11 | IS1380 | 1457/1594 | Bacteroides fragilis |
NT = Not tested.
#
Reverse-facing IS982 transposase identified using BLAST search of region upstream of cfiA, 99% identity with NCBI accession number WP_025278340.