Figure 2.

Wortmannin (Wm) inhibited basal prolactin (A), but not growth hormone (B) and luteinizing hormone (C), secretion in perfused rat anterior pituitary cells. Freshly dispersed pituitary cells were attached to beads and cultured for 20 hours before the experiments. Perfusion was performed in 0.5 ml volume chambers at a flow rate of 0.5 ml/min and the cells were washed for 2 hours before collecting samples. After the wash period, samples were collected every three minutes for 76 minutes. Vertical arrows indicate the time of application of 10 µM Wm (black circles) or solvent (white circles). Hormone content of the effluent was measured by ELISA. Data shown are representative from three (A) and two (B, C) similar experiments. For 10 µM Wm-induced inhibition of basal PRL release see also Figures 4C , 5B , C , 7C , D , 8B , and 9D .