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. 2021 Dec 30;12:790441. doi: 10.3389/fendo.2021.790441

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Copyright © 2021 Kučka, Gonzalez-Iglesias, Tomić, Prévide, Smiljanic, Sokanovic, Fletcher, Sherman, Balla and Stojilkovic

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Wm and GSK-A1 induced inhibition of PRL release are not caused by loss of intracellular PRL content and inhibition of PRL gene (Prl) expression. (A) Time course of Wm- (top) and GSK-A1 (bottom) induced inhibition of basal PRL release. (B) Time course of Wm (top) and GSK-A1 (bottom) induced accumulation of intracellular PRL content. (C) Lack of effects of Wm (top) and GSK-A1 (bottom) on Prl expression in cultured anterior pituitary cells. The experiments were performed in static incubation of cultured anterior pituitary cells: 0.25 million/well (A, B) and 1.5. million/well (C). Data points shown are mean ± SEM of sextuplicate incubation; *P < 0.002 between pairs.