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. 2021 Dec 30;12:790441. doi: 10.3389/fendo.2021.790441

Figure 8.

Figure 8

Inhibition of PI4KA by GSK-A1 causes a decrease in BayK-induced PRL release downstream of facilitated voltage-gated calcium influx. (A) BayK-stimulated voltage-gated calcium influx (left) and lack of effect of GSK-A1 on the BayK-induced rise in [Ca2+]i (right). Time of addition of TRH and Dopamine (DA) is indicated by the arrows. Traces shown are representative from three independent experiments. (B, left) In cells treated with GSK-A1, basal PRL release was progressively reduced and BayK was unable to fully restore calcium–secretion coupling. Mean ± SEM values (ng/ml) during 20 min application: BayK 186 ± 12 vs. GSK-A1+BayK = 14 ± 1; P < 0.0001. (B, right), When Wm and Bay K were applied together, the initial increase in PRL secretion was comparable to that of BayK-induced PRL release; mean ± SEM values (ng/ml) during 20 min application were 182 ± 11 (w/o Wm) vs. 176 ± 10 (with Wm). However, in Wm-treated cells, BayK-stimulated PRL release gradually declined, with a rate comparable to that of the inhibition of basal PRL release shown in the left panel, in contrast to control cells.