Table 1.
Lattice parameters for select 13-pf MT structures
| Sample | Lattice spacing/dimer rise (Å) | Skew/twist (°) | Intradimer rise (Å) | Interdimer rise (Å) | Reference |
| GMPCPP-TPX2 | 84.33 | +0.40 | 41.67 | 42.61 | (9) |
| GMPCPP | 83.95 | +0.23 | 41.53 | 42.43 | (9) |
| GMPCPP-DCX | 83.74 | +0.16 | NA | NA | (10) |
| GMPCPP-Kinesin | 83.16 | +0.19 | 41.45 | 42.00 | (9) |
| GTP-E254N | 83.45 | +0.13 | 41.53 | 41.93 | This study |
| GTP-E254A 3-start | 83.18 | −0.22 | 41.15 | 42.36 | This study |
| GTP-E254A 4-start | 83.03 | +2.40 | NA | NA | This study |
| GDP-Pi-DCX | 81.74 | 0.00 | NA | NA | (10) |
| GTPγS-Kinesin-EB3 | 81.96 | −0.28 | 41.31 | 40.51 | (9) |
| GTPγS | 81.93 | −0.12 | 41.35 | 40.45 | (9) |
| GTPγS-EB3 | 81.52 | −0.25 | 41.30 | 40.30 | (8) |
| GTPγS-DCX | 82.10 | −0.02 | NA | NA | (10) |
| GDP-DCX | 81.90 | +0.08 | NA | NA | (10) |
| GDP | 81.76 | +0.08 | 41.44 | 40.30 | (9) |
| EB3-GMPCPP | 81.71 | −0.10 | 41.50 | 40.30 | (8) |
| wt E254-GDP-Kinesin | 81.69 | −0.09 | 41.38 | 40.37 | This study |
| GTP-EB3+E254A | 81.64 | −0.19 | 41.25 | 40.44 | This study |
| GDP-Kinesin | 81.64 | +0.01 | 41.38 | 40.36 | (9) |
| GDP-Kinesin-EB3 | 81.59 | −0.11 | 41.35 | 40.32 | (9) |
| wt E254-GDP | 81.50 | +0.05 | 41.25 | 40.30 | This study |
| GDP-EB3 | 81.44 | −0.12 | 41.40 | 40.30 | (8) |
| GTP-E254N+EB3 | 81.15 | −0.29 | 41.35 | 40.45 | This study |
MT lattice parameters obtained in this study are reported alongside previously published values for reference. Expanded lattices are in blue (GMPCPP and undecorated catalytically inactive mutants), presumed transition state MTs (GDP+Pi or GTPγS) are colored in green, and compacted lattices are shaded in red (GDP state or catalytically inactive mutants + EB3). NA signifies cases in which intradimer or interdimer values were not reported due to mixing of α/β-tubulin subunits or in which resolution was too low to accurately determine the values.