. 2022 Jan 7;119(2):e2114994119. doi: 10.1073/pnas.2114994119

Table 2.

Buffers for the purification of recombinant tubulin

Buffer	Composition
Lysis	80 mM PIPES, 1 mM EGTA, 6 mM MgCl₂, 50 mM imidazole, 100 mM KCl, 2 mM GTP, 1 mM BME, and pH 7.2 + protease inhibitors and DNase
Dilution	80 mM PIPES, 1 mM EGTA, 6 mM MgCl₂, 50 mM imidazole, 2 mM GTP, 1 mM BME, and pH 7.2
His elution	80 mM PIPES, 1 mM EGTA, 5 mM MgCl₂, 500 mM imidazole, 2 mM GTP, 1 mM BME, and pH 7.2
Strep binding	80 mM PIPES, 1 mM EGTA, 5 mM MgCl₂, 2 mM GTP, mM BME, and pH 7.2
Strep elution	80 mM PIPES, 1 mM EGTA, 4 mM MgCl₂, 2.5 mM D-desthiobiotin, 50 mM imidazole, 2 mM GTP, 1 mM BME, and pH 7.2
Storage	80 mM PIPES, 1 mM EGTA, 1 mM MgCl₂, 0.2 mM GTP, and pH 6.8