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Buffers for the purification of monomeric EB3
| Lysis | 50 mM Tris pH 8.0, 150 mM NaCl, 1 mM EDTA, 1 mM DTT + protease inhibitors, DNase, RNase, and Lysozyme |
| His elution | 50 mM Tris pH 8.0, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, and 300 mM imidazole |
| SEC buffer | 50 mM Tris pH 8.0, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, and 10% glycerol |
