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Buffers for the purification of kinesin
| Lysis | 50 mM NaPO4 pH 8.0, 300 mM KCl, 2 mM MgCl2, 10% glycerol, 10 mM Imidazole, and 1 mM DTT |
| Wash | 50 mM NaPO4 pH 8.0, 150 mM KCl, 2 mM MgCl2, 10% glycerol, 30 mM Imidazole, 1 mM DTT, and 200 µM ATP |
| Elution | 50 mM NaPO4 pH 8.0, 150 mM KCl, 2 mM MgCl2, 10% glycerol, 250 mM Imidazole, and 1 mM DTT |
| SEC buffer | 25 mM Tris pH 7.5, 150 mM KCl, 10% glycerol, 2 mM MgCl2, and 2 mM DTT |
