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[Preprint]. 2022 Jan 10:2022.01.07.475453. [Version 1] doi: 10.1101/2022.01.07.475453

Figure 2. White matter microglial activation after mild respiratory SARS-CoV-2 infection.

Figure 2.

(A and B) Activated microglia (IBA1+ CD68+) quantification 7-days post-infection in the cingulum of the corpus callosum of CD1 (A) and BALB/c (B) mice. n=5 mice per CD1 control group; n=4 mice per CD1 mild COVID group; n=3 mice per BALB/c control group; n=5 mice per BALB/c mild COVID group.

(C) Representative confocal micrographs of activated microglia (IBA1, white; CD68, magenta) in the cingulum of the corpus callosum of BALB/c mice 7-days post-infection.

(D and E) Activated microglia (IBA1+ CD68+) quantification 7-weeks post-infection in the cingulum of the corpus callosum of CD1 (D) and BALB/c (E) mice. n=7 mice per CD1 control group; n=7 mice per CD1 mild COVID group; n=5 mice per BALB/c control group; n=5 mice per BALB/c mild COVID group.

(F) Representative confocal micrographs of activated microglia (IBA1, white; CD68, magenta) in the cingulum of the corpus callosum of BALB/c mice 7-weeks post-infection.

(G and H) Activated microglia (IBA1+ CD68+) quantification 7-days post-infection in the cortical gray matter of CD1 (G) and BALB/c (H) mice. n=5 mice per CD1 control group; n=4 mice per CD1 mild COVID group; n=3 mice per BALB/c control group; n=5 mice per BALB/c mild COVID group.

(I) Representative confocal micrographs of activated microglia (IBA1, white; CD68, magenta) in the cortical gray matter of BALB/c mice 7-days post-infection.

(J and K) Activated microglia quantification (IBA1+ CD68+) 7-weeks post-infection in the cortical gray matter of CD1 (J) and BALB/c (K) mice. n=7 mice per CD1 control group; n=7 mice per CD1 mild COVID group; n=5 mice per BALB/c control group; n=5 mice per BALB/c mild COVID group.

(L) Representative confocal micrographs of activated microglia (IBA1, white; CD68, magenta) in the cortical gray matter of BALB/c mice 7-weeks post-infection.

Data shown as mean +/− SEM; each dot represents an individual mouse; P values shown on figure panels; ns p>0.05 by two-tailed, unpaired t-test. Scale bars 50μm.