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. Author manuscript; available in PMC: 2022 Jan 18.
Published in final edited form as: Nat Microbiol. 2021 Jun 28;6(7):910–920. doi: 10.1038/s41564-021-00924-w

Figure 6. CrvB is required for CrvA activity in E. coli and CrvA localization drives shape dynamics in V. cholerae.

Figure 6.

Representative images from anhydrotetracycline (tet) overexpression of CrvAB in E. coli. (B) Quantification of populations from A. n.s.=confidence <95%; *=confidence >95%; **=confidence >99%; ***=confidence >99.9% (see Supplementary Table 3 for exact p-values); two-sided Wilcoxon rank sum test with Bonferroni correction; n=100 from two biological replicates (C) Dynamics of V. cholerae mean curvature (±bootstrapped 95% confidence intervals) and CrvA-GFP filament assembly after dilution from saturated overnight cultures ± 95% confidence intervals. Assembly is the Agresti-Coull estimate of percent of population with assembled structures (p˜) and its 95% confidence interval. n=300 for each point from three biological replicates. (A) Images represent 95th percentile of curvature in respective populations. (A,C) Scale bars are 1μm; images within each figure panel are to scale.