Table 1.

Comparison of the Ct values between the singleplex and multiplex qRT-PCR

Plasmid	Concentration (copies/μL)	2.52 × 107	2.52 × 106	2.52 × 105	2.52 × 104	2.52 × 103	2.52 × 102	2.52 × 101	2.52 × 100	2.52 × 10−1
p-ASFV	Singleplex qRT-PCR	13.781	17.478	20.347	24.044	28.274	31.010	35.073	39.481	–
	Multiplex qRT-PCR	13.846	16.859	20.052	24.052	27.862	31.227	34.776	39.351	–
p-CSFV	Singleplex qRT-PCR	13.490	17.195	20.329	24.584	27.974	31.427	34.793	39.341	–
	Multiplex qRT-PCR	13.788	17.011	20.433	24.418	28.270	31.837	35.074	39.857	–
p-APPV	Singleplex qRT-PCR	12.172	15.430	19.161	23.145	27.183	30.502	33.480	36.519	–
	Multiplex qRT-PCR	11.976	15.469	18.845	22.955	26.767	30.154	34.328	36.830	–