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. 2022 Jan 18;21:23. doi: 10.1186/s12943-022-01497-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — CircMET is significantly high-expressed in NONO-TFE3 tRCC. (A) The RNA level of circMET (red) in NONO-TFE3 tRCC and ccRCC was detected by FISH. Nuclei were visualized using DAPI (blue) counterstain. (B-C) cDNA and genomic DNA (gDNA) of UOK109 cells and 786-O cells were used to amplify circMET and GAPDH with divergent primers and convergent primers, respectively. (D-E) The RNA levels of circMET and MET mRNA in both UOK109 cells and 786-O cells were detected by real-time PCR after treatment of RNase R. (F-G) The expression of circMET and MET mRNA in both UOK109 cells and 786-O cells was detected by real-time PCR after treatment with α-amanitin at the indicated time points. (H-I) The subcellular distribution of circMET was analyzed via real-time PCR in ccRCC cell line (786-O and ACHN), tRCC cell lines (UOK109 and UOK120) and normal cell lines (HK-2 and HEK293T). U6 and 18s rRNA were used as nuclear and cytoplasmic markers, respectively. (J-K) The location of circMET (red) in UOK109 and 786-O cells was determined by FISH assay. U6 and 18s rRNA were used as nuclear and cytoplasmic markers, respectively. DAPI-stained nuclei are blue. (L) The RNA level of circMET was analyzed by real-time PCR assay in HEK293T, HK-2, ACHN, 786-O, UOK120 and UOK109 cells. The data are presented as the mean ± SD