Table I.
Primer sequences used for reverse transcription-quantitative PCR.
| Gene | Direction | Primer sequence (5'-3') |
|---|---|---|
| Cox-2 | Forward | ACCCGTGGAGCTCACATTAACTAT |
| Reverse | ATACTGTTCTCCGTACCTTCACCC | |
| iNOS | Forward | CACTTCCAACGCAACATGGG |
| Reverse | CTTTGACCCAGTAGCTGCCA | |
| p53 | Forward | GAGGATTCACAGTCGGAT |
| Reverse | ATCATCTGGAGGAAGAAGTT | |
| p16 | Forward | GCTGCCCAACGCACCGAATA |
| Reverse | ACCACCAGCGTGTCCAGGAA | |
| Aggrecan | Forward | TGAGCGGCAGCACTTTGAC |
| Reverse | TGAGTACAGGAGGCTTGAGG | |
| Collagen-2 | Forward | GAACTGGTGGAGCAGCAAGA |
| Reverse | AGCAGGCGTAGGAAGGTCAT | |
| GAPDH | Forward | CCACCAACTGCTTAGCCCCC |
| Reverse | GCAGTGATGGCATGGACTGTGG |
Cox-2, cyclooxygenase 2; iNOS, inducible nitric oxide synthase.