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. 2021 Dec 30;23(2):182. doi: 10.3892/etm.2021.11105

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of HSYA on tert-butyl hydroperoxide-induced NP cell apoptosis. (A) Flow cytometry was used to detect the level of apoptosis. (B) Diamidino-2-phenylindole was used to stain and analyze nuclear morphology. The red circles indicate pyknotic nuclei and apoptotic bodies. Scale bar, 50 µm. (C) Statistical analysis of apoptosis detection using flow cytometry. (D) Western blot analysis was used to detect the expression level of apoptosis-related proteins and the results were statistically analyzed for (E) Bcl-2, Bax and (F) cleaved caspase-3 and cleaved caspase-9. ^*P<0.05, ^**P<0.01 vs. control; ^##P<0.01 vs. model. HSYA, hydroxysafflor yellow A.