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. 2022 Jan 14;219(2):e20210815. doi: 10.1084/jem.20210815

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© 2022 Kerdidani et al.

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Figure S2. — Lung CAF specificity of the Col1a2-CreER, ColVI-Cre, and Twist2-Cre mouse strains. (A and B) LLC cells were implanted intrapulmonary in the left lung lobe of tamoxiphen-treated Col1a2-CreER/mTmG (Col1a2), ColVI-Cre/mTmG (ColVI), and Twist2-Cre/mTmG mice (Twist) mice. Lung tumors were digested and subjected to FACS analyses. (A) Expression of hematopoietic- (CD45), endothelial- (CD31), and epithelial- (EpCAM) specific markers by lung GFP cells. (B) Twist2 expression of the fibroblast-specific marker Podoplanin and of IAb (MHCII) (n = 2 per group, from two independent experiments). FMO, fluorescence minus one.