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. 2022 Jan 14;219(2):e20210815. doi: 10.1084/jem.20210815

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© 2022 Kerdidani et al.

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Figure S3. — Lysosomal processes of MHCII-deleted apCAFs. Purified apCAFs from I-Ab-fl/fl mice were expanded and transduced with CRE^mcherry or control (CTL) lentiviruses. To induce MHCII, apCAFs were exposed for 72 h in 3D conditions to 30% tumor homogenate (Tumor_Homo). Left: Representative FACS plots of MHCII. Lysosomes were tracked (LysoTracker), and lysosomal proteolysis was assessed (DQ Green BSA) with FACS. Right: Representative FACS plots. FSC-A, forward scatter-A; FMO, fluorescence minus one.