Figure 4. Overexpression of soluble PD-L1 variants did not help the tumor growth alone.

(A) Mice were injected with 1 × 10⁶ MC38 cells transduced with empty vector (n = 6). (B) MC38_PD-L1–KO cells transduced with empty vector (n = 6). (C) MC38_PD-L1–KO cells transduced with murine WT PD-L1 (n = 6). (D) MC38_PD-L1–KO cells transduced with mh #1 (n = 6). (E) MC38_PD-L1–KO cells transduced with mh #2 (n = 6). (F) MC38_PD-L1–KO cells transduced with mm (n = 6). Each line represents the tumor volumes for each mouse. mh #1 and #2 are different clones. Each group was compared with 2-sided Mann-Whitney U test.