Figure 10. Impact of neonatal GCV treatment on neuroimaging abnormalities and brain calcification.

(A) Neuroimaging by CT after GCV treatment. Neuroimaging by CT was applied to assess brain damage of mice at P14. Representative CT images with cerebral calcification outlined by white dashed lines are shown. The calcification volume was estimated using imaging software. The percentage of calcification volume relative to that of the MCMV group was calculated. Data were analyzed by 2-tailed Student’s t test and results are presented as means ± SEMs. **P < 0.01. Scale bar: 2 mm. (B) GCV treatment, tissue loss, and cerebral calcification by IHC. Position-matched adjacent sequential coronal cerebral sections of mice in each group at P14 were stained with HE for brain architecture and with von Kossa and Alizarin red for cerebral calcium deposition. Scale bar: 1 mm; 50 μm (magnified images).