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. Author manuscript; available in PMC: 2022 Sep 11.
Published in final edited form as: J Vis Exp. 2021 Sep 11;(175):10.3791/62400. doi: 10.3791/62400

Figure 1 –

Figure 1 –

Representation of exocytic analysis and classification. A) Outline of the analysis pipeline for the GUI. Cells are segmented from the background before exocytic events are identified and tracked. Parameters such as the peak ΔF/F and t1/2 are calculated from fluorescent traces of exocytosis in a ROI around the event pre and post fusion. B) illustration of the four modes of exocytosis and example image montages. After fusion, events may proceed instantaneous to FVF or KNR (FVFi and KNRi), or a delay may be present before onset of fusion fate to FVF or KNR (FVFd and KNRd).