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. 2022 Jan 18;66(1):e01875-21. doi: 10.1128/AAC.01875-21

FIG 4.

FIG 4

Evaluation of the role of SDH, PMF and intracellular pH on the potentiating effect of additional carbon source on antibiotic during eradication of P. aeruginosa PAO1 biofilms. 24-h-old-biofilm growth treated by antibiotic (or not) in SCFM2 supplemented with or without additional carbon source in the presence or absence of (A) the SDH inhibitor TTFA, (B) the proton motive force disruptor CCCP, (C) the K+ ionophore nigericin and excess levels of K+. MA: 60 mM d,l-malic acid; CIP: 1.2 μg/ml ciprofloxacin; MA-CIP: 60 mM d,l-malic acid combined with 1.2 μg/ml ciprofloxacin; SA: 120 mM sodium acetate; CAZ: 12.48 μg/ml ceftazidime; SA-CAZ: combination of 120 mM sodium acetate and 12.48 μg/ml ceftazidime. Values shown are the mean (n ≥ 3). Error bars indicate standard error, **, P < 0.01; ***, P ≤ 0.001; ****, P < 0.0001.