Table 3.

Reaction scheme.

Assay description	Negative control	Positive control	Inhibition
Fluorescent assay buffer	80 µL	78 µL	78-X µL
BACE1 substrate solution, 50 µM	20 µL	20 µL	20 µL
BACE1 enzyme solution, 0.3 unit/µL	–	2 µL	2 µL
Inhibitor solution	–	–	X µL
Total	100 µL	100 µL	100 µL