TABLE 7.
Inhibitor binding constants calculated for the Cdr1 ATPase activities of wt Cdr1 and milbemycin α25-resistant Cdr1 mutants
| Strain |
Kia (μM) of: |
||||
|---|---|---|---|---|---|
| FK506 | Enniatin B | Beauvericin | Oligomycin | Vanadate | |
| CDR1 | 1.21 ± 0.24 | 0.20 ± 0.03 | 0.08 ± 0.01 | 0.54 ± 0.01 | 0.96 ± 0.08 |
| G521R | 12.8 ± 4.53 | 5.34 ± 0.41 | 2.34 ± 0.20 | 2.54 ± 0.24 | 20.8 ± 0.76 |
| M639I | 0.56 ± 0.07 | 0.48 ± 0.04 | 0.08 ± 0.02 | 1.15 ± 0.12 | 1.93 ± 0.13 |
| A713P | 11.9 ± 2.8 | NAb | NA | 0.43 ± 0.06 | 0.07 ± 0.01 |
| T1355N | 0.29 ± 0.02 | 0.09 ± 0.06 | 0.05 ± 0.03 | 0.22 ± 0.05 | 2.21 ± 0.46 |
a
The Ki values were calculated using the kinetic results presented in Table 6.
b
NA, not applicable because enniatin B and beauvericin induce the ATPase activity of A713P.