FIG 5.

Three antisense RNA coverage hot spots in P. aeruginosa NN2 during mid-exponential (upper) and early stationary (lower) growth. Antisense RNA reads of the biological replicates 1 (black), 2 (yellow), and 3 (light blue) were aligned to the genome sequence with minimap2. Green and orange arrows represent read alignments toward coding sequences with no functional annotation (CDS, undefined) and known coding sequences, respectively. The dark blue label depicts tRNA coverage. The abscissa provides the map position of the selected genomic region. Please note that the bead-based purification of RNA prior to sequencing introduced a skew toward shorter and more heterogeneous read lengths.