FIG 2.

(A and B) Expression of SigB-regulated genes in different genetic backgrounds. Northern blot analysis showing expression levels of positively (lmo2230 and lmo0596) and negatively (lmo1699) SigB-regulated genes. The strains (WT, ΔsigB, ΔrsbX, and ΔrsbX + rsbX) were grown at 37°C (A) or 23°C (B) in BHI medium in darkness to prevent light-induced stress, with constant agitation (180 rpm). Samples were taken when cultures reached an OD₆₀₀ of ∼0.8, and the RNA was extracted. tmRNA was used as a loading control. n = 3. (C and D) Western blot analysis determining levels of SigB. WT, ΔsigB, ΔrsbX, and ΔrsbX + rsbX strains were grown in BHI at 37°C (C) or 23°C (D) in BHI medium in darkness with constant agitation until OD₆₀₀ of ∼0.8 was reached. Samples were taken and protein extracted before Western blot analysis using anti-SigB antibodies. GroEL levels were used as a loading control; n = 3.