TABLE 2.
Oligonucleotides used in this study
| Oligonucleotide sequence (5′–3′) | Target |
|---|---|
| Cloning | |
| GGGGGATCCGGGGAGATCGATGCTTATACAGCG | sigB, rsbX a fwd (BamHI restriction site underlined) |
| GGGGTCGACTTTATCAGGTTGAGATACTTTTGGC | sigB, rsbX b rev (SalI restriction site underlined) |
| GGGGTCGACTAACCATAACACCTTTTAAACTAGCG | sigB, rsbX c fwd (SalI restriction site underlined) |
| GGGCCATGGCGTAACCACAAGTCCAATAAGTGG | sigB, rsbX d rev (NcoI restriction site underlined) |
| AGTCCCAATATAATCATTTATCAACTC | pMAD_seq F |
| TGGTCGTCATCTACCTG | pMAD_seq R |
| GGGGGATCCTGAGGAAGTGGAGTAAATG | Comp rsbX fwd (BamHI restriction site underlined) |
| GGGCTGCAGTGTTCTCACACCCAATCTG | Comp rsbX rev (PstI restriction site underlined) |
| Northern blotting | |
| CAATGAGATCAGCAT | lmo2230 L |
| GCATATTCGAAGTGC | lmo2230 U |
| CCTCCACATTAATGTTTGG | lmo0596 fwd |
| GCAAGAAACCTAGTAAGATGG | lmo0596 rev |
| CGCAACTCTCCTTCTCGATGG | lmo1699 fwd |
| GCTTTATAGCTCGCACTTTGG | lmo1699 rev |
| CCTCGTTATCAACGTCAAAGCC | tmRNA fwd |
| CGGCACTTAAATATCTACGAGC | tmRNA rev |
| RT-qPCR | |
| CATATTCGAAGTGCCATTGC | lmo2230_F |
| CTGAACTAGGTGAATAAGACAAAC | lmo2230_R |
| GGGTACTAGCTGACGGAATTTTATC | lmo0596_F |
| CCCACATACCGAAAAGTAATACGAG | lmo0596_R |