FIG 2.

Electropherograms of fluorescent dye-labeled DNA fragments. (a and b) Reduced electropherogram signals indicate protection regions by RrpA_his6 upstream of mdaB (a) and rrpA (b). (c) Protection regions by RrpB_his6 upstream of rrpB. (Regions between the lines indicate protected regions; blue and green electropherograms indicate FAM and Hex fluorescently labeled DNA fragments, respectively. The electropherograms are presented as arbitrary scale). (d and e) The protected regions by RrpA_his6 (gray highlight) including the inverted repeat sequence (bold and underlined) upstream of rrpA and mdaB transcription start sites. (f) The protected region by RrpB_his6 (gray highlight) upstream of rrpB (inverted repeat sequences are bold and underlined). (g) The sequence alignment of the rrpB protected region and the region upstream of nfrA are matched (asterisks). Nucleotides highlighted in green indicate the C. jejuni ribosomal biding site (70), and yellow highlights indicate the gene translation start site. Electropherograms are representative of three experiments.