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. 2022 Jan 18;204(1):e00421-21. doi: 10.1128/JB.00421-21

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Copyright © 2022 Nasher et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Electrophoretic mobility shift assay. The EMSA was conducted to confirm RrpA_his6 and RrpB_his6 DNA binding activity. (a and b) RrpA_his6 (0.05 μg) binds upstream of mdaB (a), and RrpB_his6 (0.05 μg) binds upstream of nfrA (b). (c and d) RrpA_his6 binds upstream of rrpA (c), and RrpB_his6 binds upstream of rrpB (d). Binding is indicated by a shift of band (arrows). IRDye 800 DNA fragments were used and imaged with LI-COR Odyssey imaging scanner (LI-COR Biosciences).