TABLE 2.
PTM identification following database searching and manual verification of the matched fragmentation spectra for RrpAhis6 and RrpBhis6 proteinsa
| Sample | Peptide | m/z | Charge | Mass (Da) | Residue(s) | Total ion current (TIC) intensity |
|---|---|---|---|---|---|---|
| RrpA (control) | 4ENSPCDhaNFEECdioxGFNYTLALINGK25 | 821.03 | 3 | 2,460.1 | Cys8; Cys13 | 208,300 |
| 28MSILYCDhaLFR36 | 556.30 | 2 | 1,110.6 | Cys33 | 204,200 | |
| 28MoxSILYCDhaLFR36 | 564.30 | 2 | 1,126.6 | Met28; Cys33 | 110,600 | |
| RrpA (2-hydroxy-1,4-naphthoquinone) | 4ENSPCDhaNFEECdioxGFNYTLALINGK25 | 821.03 | 3 | 2,460.1 | Cys8; Cys13 | 1,119,000 |
| 28MSILYCDhaLFR36 | 556.30 | 2 | 1,110.6 | Cys33 | 877,900 | |
| RrpA (H2O2) | 4ENSPCDhaNFEECdioxGFNYTLALINGK25 | 821.03 | 3 | 2,460.1 | Cys8; Cys13 | 849,400 |
| 28MoxSILYCDhaLFR36 | 564.30 | 2 | 1,126.6 | Met28; Cys33 | 259,400 | |
| RrpB (control) | 4YHSLCsulfdioxPIETTLNLIGNK20 | 990.48 | 2 | 1,979 | Cys8 | 37,140 |
| RrpB (H2O2) | 4YHSLCsulfdioxPIETTLNLIGNK20 | 990.48 | 2 | 1,979 | Cys8 | 82,780 |
a
The complete data set is presented in Table S2.