FIG 3.
TolQ-dependent recruitment of TolAI (TolA1-60) to division sites. Fluorescence (left) and DIC (right) images of lysogenic derivatives of strains LP49 (ΔtolA) (A), LP51 (Δ[tolR-pal]) (B), and LP57 (Δ[tolQ-cpoB]) (C and D), carrying λDE3 (Plac::gfp-tolA1-60) integrated in the chromosome. The cells in panel D also harbored plasmid pLP146 (PBAD::tolQ). Cells were grown for ∼3.5 mass doublings to OD600 = 0.5 to 0.6 in M9-maltose medium with 37 μM IPTG and either no (A to C) or 0.02% (D) arabinose and were imaged live. Bar equals 2 μm.