FIG 4.
Recruitment of TolAIII (TolA294-421) to division sites does not require TolQ or TolR. Fluorescence (left) and DIC (right) images of lysogenic derivatives of strains LP49 (ΔtolA) (A and B), LP56 (Δ[tolQ-tolA]) (C), and LP50 (Δ[tolA-pal]) (D), carrying λCH543 (Plac::gfp-malF2-39-rodZ139-255) (A), or λCH536 (Plac::gfp-malF2-39-rodZ139-255-tolA294-421) (B to D) integrated in the chromosome. Cells were grown for ∼3.5 mass doublings to OD600 = 0.5 to 0.6 in M9-maltose medium with 37 μM IPTG and imaged live. Bar equals 2 μm.