FIG 5.

Efficient recruitment of TolAIII (TolA^294-421) to division sites requires TolB, Pal, and CpoB. Fluorescence (left) and DIC (right) images of strain LP57 (Δ[tolQ-cpoB]) carrying λCH543 (P_lac::gfp-malF^2-39-rodZ^139-255) (A), or λCH536 (P_lac::gfp-malF^2-39-rodZ^139-255-tolA^294-421) (B to E) integrated in the chromosome, and harboring plasmid pCH528 (P_BAD::tolB pal cpoB) (A and B), pCH518 (P_BAD::tolB pal) (C), pCH545 (P_BAD::pal cpoB) (D), or pCH544 (P_BAD::tolB pal⁰ cpoB) (E). Cells were grown for ∼3.5 mass doublings to OD₆₀₀ = 0.5 to 0.6 in M9-maltose medium with 37 μM IPTG and imaged live. Bar equals 2 μm. Plasmid pCH544 is identical to pCH528, except for a frameshift at codon 53 of the Pal open reading frame (ORF). The arrowhead in panel E points at a rare septal accumulation of the GFP-M-L-TolAIII fusion that was seen in this population of cells at an approximately 6-fold lower frequency than that of panel B (see Table S2).