TABLE 1.

Localization of TolA derivatives in the absence of other Tol-Pal proteins and CpoB^a

GFP fusionb			% of cells with accumulationc			n d
Phage	Residues	TolA domain(s)	+ +	+ −	− −
λNP4	-TolA1-421	I+II+III	49	11	40	191
λDE2	-TolA1-328	I+II	35	12	53	321
λCH512	-TolA1-292	I+II'	48	10	42	503
λCH483	-TolA1-111	I	4	12	84	348
λDE3	-TolA1-60	I	1	5	94	364
λCH509	-MalF2-39-TolA47-421	II+III	35	13	52	372
λCH510	-MalF2-39-TolA47-328	II	34	15	51	282
λCH549	-MalF2-39-TolA47-292	II'	34	14	52	228
λCH536	-MalF2-39-RodZ139-255-TolA294-421	III	0	1	99	528

^aLP57(Δ[tolQ-cpoB]) cells lysogenic for the indicated phage were grown for ∼3.5 mass doublings to OD₆₀₀ = 0.5 to 0.6 in M9-maltose medium with IPTG and imaged live. IPTG was used at 25 or 37 μM for each lysogen, and the results from both conditions were combined.

^bIndicated are the name of the lysogenic phage encoding the fusion under the control of the lac regulatory region, the TolA residues encoded, and the presence of intact TolAI (TolA^1-42), TolAII (TolA^48-310), and/or TolAIII (TolA^314-421) domains in the fusion (48). II' indicates that the encoded TolAII domain is slightly truncated at its C-terminal end. GFP is N terminal in all cases. MalF^2-39 includes the first transmembrane helix of MalF (MalF^19-35) (89). RodZ^139-255 corresponds to the periplasmic linker domain of the RodZ protein (90).

^cPercentage of cells in which the GFP fusion accumulated strongly (+ +) or weakly (+ −) at sites of cell constriction or appeared evenly distributed along the periphery of cells (− −).

^dNumber of cells scored.